Anti-OTUD5 Antibody

About this product

Boster Bio Anti-OTUD5 Antibody (Catalog # A09744) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A09744

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

OTUD5 is a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and OTUD5 has been shown to suppress the type I interferon (IFN-I) -dependent innate immune response by cleaving the polyubiquitin chain from TRAF3, an essential type I interferon adaptor protein. Cleavage results in disassociation of TRAF3 from a downstream signaling complex containing TBK1 and the disruption of the IFN-I signaling cascade, indicating that OTUD5 acts as a negative regulator of innate immune responses. It has been suggested that by suppressing IFN-I production, OTUD5 may function to inhibit the emergence of certain autoimmune disorders such as systemic lupus erythematosus. Multiple isoforms of OTUD5 are known to exist.

Synonyms

DUBA, OTU domain-containing protein 5, Deubiquitinating enzyme A, DUBA

Gene Name

OTUD5

UniProt

Q96G74

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

OTUD5 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human OTUD5. The immunogen is located within amino acids 400 - 450 of OTUD5.

Clonality

Polyclonal

Tissue Specificity

Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Innate Immunity

Purification

OTUD5 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'- linked ubiquitin chains (in vitro) .

Storage Conditions

OTUD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

60626 MW

Gene Name Synonym

OTU domain containing 5

Subcellular Location

Cell membrane; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl- 2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.

Protein Name

OTU domain containing 5

Isotype

IgG

Contents

OTUD5 Antibody is supplied in PBS containing 0.02% sodium azide.