Anti-HAAO Antibody

About this product

Boster Bio Anti-HAAO Antibody (Catalog # A10482) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A10482

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

HAAO (3-Hydroxyanthranilate 3, 4-dioxygenase) is a monomeric cytosolic protein of the family of intramolecular dioxygenases containing non-heme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is present in low amounts in the central nervous system. This enzyme participates in tryptophan metabolism. It employs one cofactor, iron. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurological and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. Furthermore, recent study shows that HAAO are excellent candidate biomarkers for detecting ovarian cancer.

Synonyms

HAO, 3-HAO, 3-hydroxyanthranilate 3, 4-dioxygenase, 3-hydroxyanthranilate oxygenase

Gene Name

HAAO

UniProt

P46952

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HAAO antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human HAAO. The immunogen is located within amino acids 30 - 80 of HAAO.

Clonality

Polyclonal

Applications

ELISA, WB, IHC-P, IF

Field of Research

Homeostasis

Purification

HAAO Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Catalyzes the oxidative ring opening of 3- hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Storage Conditions

HAAO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

32556 MW

Applications Notes

HAAO antibody can be used for detection of HAAO by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Gene Name Synonym

3-hydroxyanthranilate 3,4-dioxygenase

Subcellular Location

Cytoplasm, cytosol

Prediction Reactivity

Bovine

Protein Name

3-hydroxyanthranilate 3,4-dioxygenase

Isotype

IgG

Contents

HAAO Antibody is supplied in PBS containing 0.02% sodium azide.