Anti-Histone H4 HIST1H4A Antibody

About this product

Boster Bio Anti-Histone H4 HIST1H4A Antibody (Catalog # A14495) . Tested in Dot blot, WB applications. This antibody reacts with Human.

Specifications

Catalog number

A14495

Size / count

100 µL

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Synonyms

Histone H4, Histone H4, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4J, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Gene Name

HIST1H4A

UniProt

P62805

Host

Rabbit

Reactivity

Human

Immunogen

Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.

Clonality

Polyclonal

Applications

ELISA, WB

Field of Research

2339, Epigenetics and Nuclear Signaling, Histones, Unmodified

Purification

Anti-Histone H4 pan Antibody antiserum is directed against Histone H4. Cross-reactivity with other species was not tested.

Concentration

90 mg/mL by Refractometry

Form

Liquid (sterile filtered)

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

11367 MW

Gene Name Synonym

Histone H4

Subcellular Location

Nucleus. Chromosome.

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide