Anti-SGOL2 Antibody (Center)

About this product

Boster Bio Anti-SGOL2 Antibody (Center) (Catalog # A30763-1) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

A30763-1-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity) . Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.

Synonyms

Shugoshin-like 2, Shugoshin-2, Sgo2, Tripin, SGOL2

Gene Name

SGO2

UniProt

Q562F6

Host

Rabbit

Reactivity

Human

Immunogen

This SGOL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids from the Central region of human SGOL2.

Clonality

Polyclonal

Applications

WB

Field of Research

Cell Biology

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity) . Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments. Involved in centromeric enrichment of AUKRB in prometaphase.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

144739 Da

Applications Notes

WB: 1:1000

Subcellular Location

Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q7TSY8}. Note=During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres (By similarity) . SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost (By similarity) . During meiosis II, it shows a striking tension- dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (By similarity) . In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase (PubMed:17485487) Centromeric localization requires the presence of BUB1 and AUKRB (PubMed:17485487) . {ECO:0000250|UniProtKB:Q7TSY8, ECO:0000269|PubMed:17485487}

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.