Anti-ATP5B Antibody (Center)

About this product

Boster Bio Anti-ATP5B Antibody (Center) (Catalog # A32270-2) . Tested in IHC-P, IF, Flow Cytometry, WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

A32270-2-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . It is the beta subunit of the catalytic core.

Synonyms

ATP synthase subunit beta, mitochondrial, ATP5B, ATPMB, ATPSB

Gene Name

ATP5F1B

UniProt

P06576

Host

Rabbit

Reactivity

Human

Immunogen

This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.

Clonality

Polyclonal

Applications

IHC-P, IF, Flow Cytometry, WB

Field of Research

Metabolism, Signal Transduction, Mitochondria

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1) . Rotation of the central stalk against the surrounding alpha (3) beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

56560 Da

Subcellular Location

Mitochondrion inner membrane {ECO:0000250|UniProtKB:P00829}; Peripheral membrane protein {ECO:0000250|UniProtKB:P00829}; Matrix side {ECO:0000250|UniProtKB:P00829, ECO:0000269|PubMed:25168243}

Prediction Reactivity

Bovine, Mouse, Rat

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.