Anti-ATP5J Antibody (Center)

About this product

Boster Bio Anti-ATP5J Antibody (Center) (Catalog # A32371-1) . Tested in WB, IF, IHC-P application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A32371-1-80ul

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.

Synonyms

ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, ATP5J, ATP5A, ATPM

Gene Name

ATP5PF

UniProt

P18859

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.

Clonality

Polyclonal

Applications

WB, IF, IHC-P

Field of Research

Metabolism, Signal Transduction, Mitochondria

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3) beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

12588 Da

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Prediction Reactivity

Monkey

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.