Anti-Phospho-beta-catenin (Ser33/37) Antibody

About this product

Boster Bio Anti-Phospho-beta-catenin (Ser33/37) Antibody (Catalog # M00004-4) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

M00004-4-200ul

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

1752450318077326180868581895742321262353226473782269993822155184

Synonyms

Catenin beta-1, Beta-catenin, CTNNB1, CTNNB

Gene Name

CTNNB1

UniProt

P35222

Host

Rabbit

Reactivity

Human

Immunogen

This Phospho-beta-catenin (Ser33/37) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-41 amino acids from human beta-catenin .

Clonality

Polyclonal

Tissue Specificity

Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level) . Expressed in breast cancer tissues (at protein level) (PubMed:29367600) .

Applications

WB

Field of Research

Cancer, Cardiovascular, Neuroscience, Signal Transduction, Stem Cells, Anti-Wnt-Pathway (plus Lgr5) Antibodies

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

85497 Da

Applications Notes

WB: 1:1000

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:B6V8E6}. Cell junction, adherens junction. Cell junction {ECO:0000250|UniProtKB:B6V8E6}. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Synapse {ECO:0000250|UniProtKB:Q02248} Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q02248}. Note=Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity) . Cytoplasmic when it is un-stable (highly phosphorylated) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation) . Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Interaction with FAM53B promotes translocation to the nucleus (PubMed:25183871) . {ECO:0000250|UniProtKB:B6V8E6, ECO:0000269|PubMed:25183871}

Prediction Reactivity

Bovine, Zebrafish, Mouse, Rat, Xenopus

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.