Anti-RAC1 Antibody

About this product

Boster Bio Anti-RAC1 Antibody (Catalog # M00041-1) . Tested in IHC-P, Flow Cytometry, WB application (s) . This antibody reacts with Human, Rat.

Specifications

Catalog number

M00041-1-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

Synonyms

Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, RAC1, TC25

Gene Name

RAC1

UniProt

P63000

Host

Mouse

Reactivity

Human, Rat

Immunogen

This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.

Clonality

Monoclonal

Clone

Clone: 1301CT276.121.104

Tissue Specificity

Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues

Applications

IHC-P, Flow Cytometry, WB

Field of Research

Cancer, Cell Biology, Immunology, Signal Transduction, Anti-Wnt-Pathway (plus Lgr5) Antibodies, TLR Signaling, T Cells, Chemokine signaling, Inflammation

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

164365828886345235121989121475164365812695502

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

21450 Da

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P63001}. Cell projection, dendrite {ECO:0000250|UniProtKB:P63001}. Synapse {ECO:0000250|UniProtKB:Q6RUV5} Nucleus. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (PubMed:1903399) . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) . Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity) . In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity) Activation by GTP-binding promotes nuclear localization (PubMed:12551911) . {ECO:0000250|UniProtKB:P63001, ECO:0000250|UniProtKB:Q6RUV5, ECO:0000269|PubMed:12551911, ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:1903399}

Prediction Reactivity

Bovine, Mouse

Isotype

IgG2b

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.