Anti-Hu CD28 Purified Monoclonal Antibody

About this product

Boster Bio Anti-Hu CD28 Purified Monoclonal Antibody (Catalog# M00065) . Tested in Flow Cytometry, IP, WB, IHC-F, ICC application (s) . This antibody reacts with Primate, Human.

Specifications

Catalog number

M00065

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Synonyms

TP44

Gene Name

CD28

UniProt

P10747

Host

Mouse

Reactivity

Primate, Human

Immunogen

DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA. The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Clonality

Monoclonal

Clone

Clone: CD28.2

Tissue Specificity

Expressed in T-cells and plasma cells, but not in less mature B-cells.

Applications

Flow Cytometry, IP, WB, IHC-F, ICC

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/mL

Form

Liquid

Function

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933) . Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037) .

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

25066 MW

Applications Notes

Flow cytometry: 1-12 μg/ml

Gene Name Synonym

CD28 molecule

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

T-cell-specific surface glycoprotein CD28

Isotype

Mouse IgG1 kappa

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide