Anti-MAPK3/1 Antibody (Center)

About this product

Boster Bio Anti-MAPK3/1 Antibody (Center) (Catalog # M00104-4) . Tested in WB, IHC-P, IF application (s) . This antibody reacts with Human, Mouse.

Specifications

Catalog number

M00104-4-200ul

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC) ; as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10) . Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Synonyms

Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, 2.7.11.24, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3

Gene Name

MAPK3

UniProt

P27361

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This MAPK3/1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 175-208 amino acids from the Central region of human MAPK3/1.

Clonality

Monoclonal

Clone

Clone: 1129CT3.4.3

Applications

WB, IHC-P, IF

Field of Research

Apoptosis, Interleukins, Inflammation, Chemokine signaling, T Cells, TLR Signaling, Host-Virus Interaction, Viral process, Stem Cells, Signal Transduction, Neuroscience, Immunology, Cell Biology, Cancer

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

3449736835216969

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

43136 Da

Subcellular Location

Cytoplasm {ECO:0000250|UniProtKB:P21708}. Nucleus. Membrane, caveola {ECO:0000250|UniProtKB:P21708}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q63844} Note=Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905) . PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity) . {ECO:0000250|UniProtKB:Q63844, ECO:0000269|PubMed:19060905}

Prediction Reactivity

Drosophila, Bovine, Rat, Xenopus

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.