Anti-PCNA Antibody

About this product

Boster Bio Anti-PCNA Antibody (Catalog # M00125-2) . Tested in WB, Flow Cytometry, IHC-P application (s) . This antibody reacts with Human.

Specifications

Catalog number

M00125-2-50ul

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Synonyms

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Gene Name

PCNA

UniProt

P12004

Host

Mouse

Reactivity

Human

Immunogen

This PCNA antibody is generated from a mouse immunized with a recombinant protein of human PCNA.

Clonality

Monoclonal

Clone

Clone: 1655CT506.10.26

Applications

WB, Flow Cytometry, IHC-P

Field of Research

Cancer, Cell Biology

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

355852322493990224695737

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

28769 Da

Subcellular Location

Nucleus Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902) . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136) . Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136) . Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.