Anti-IF4E Antibody

About this product

Boster Bio Anti-IF4E Antibody (Catalog # M00135-2) . Tested in WB, IF application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00135-2-80ul

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Synonyms

Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F

Gene Name

EIF4E

UniProt

P06730

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.

Clonality

Monoclonal

Clone

Clone: 1504CT774.14.55

Applications

WB, IF

Field of Research

Viral process, Host-Virus Interaction

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

116062002433528529987188226840102257881316271312225788132998718824335285243352852347107822684010

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

25097 Da

Subcellular Location

Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus. Nucleus speckle. Nucleus, nuclear body Note=Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732) . Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257) Sequestered in the nucleus by EIF4EBP1 and EIF4EBP2 (By similarity) {ECO:0000250|UniProtKB:P63073, ECO:0000269|PubMed:10856257, ECO:0000269|PubMed:16157702, ECO:0000269|PubMed:24335285, ECO:0000269|PubMed:25923732}

Isotype

IgG1, Κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.