Anti-p21 WAF1 CDKN1A Monoclonal Antibody

About this product

Boster Bio Anti-p21 WAF1 CDKN1A Monoclonal Antibody (Catalog # M00145) . Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M00145

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

P21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.

Synonyms

P21/WAF1, Cyclin-dependent kinase inhibitor 1A (p21, CIP1), DNA Synthesis Inhibitor, MDA 6, MDA6, Melanoma Differentiation Associated Protein 6

Gene Name

CDKN1A

UniProt

P38936

Host

Mouse

Reactivity

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

Clonality

Monoclonal

Clone

Clone: Clone: WA-1 IgG1 kappa

Tissue Specificity

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Applications

ELISA, IHC, IF, IP, WB

Field of Research

Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Chromatin Binding Proteins, Co-Activators/Co-Repressors, DNA/RNA Binding, Domain Families, Epigenetics and Nuclear Signaling, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Zinc Finger

Purification

This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1) . It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA) . The antibody is highly specific and shows no cross-reactivity with other closely related mitotic inhibitors.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

18119 MW

Gene Name Synonym

Cyclin-dependent kinase inhibitor 1

Subcellular Location

Cytoplasm. Nucleus.

Isotype

IgG1 kappa

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide