Anti-CCND1 Antibody

About this product

Boster Bio Anti-CCND1 Antibody (Catalog # M00149) . Tested in WB application (s) . This antibody reacts with Human, Mouse.

Specifications

Catalog number

M00149-200ul

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

Synonyms

G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

Gene Name

CCND1

UniProt

P24385

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This antibody is generated from a mouse immunized with a recombinant protein from human.

Clonality

Monoclonal

Clone

Clone: 1849CT557.93.5

Applications

WB

Field of Research

Cancer, Cell Biology, Anti-Wnt-Pathway (plus Lgr5) Antibodies

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

33729 Da

Applications Notes

WB: 1:2000

Subcellular Location

Nucleus. Cytoplasm Nucleus membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members

Prediction Reactivity

Human

Isotype

IgG1, κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.