Anti-IRF3 Antibody (N-Term)

About this product

Boster Bio Anti-IRF3 Antibody (N-Term) (Catalog # M00165-5) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

M00165-5-50ul

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Key transcriptional regulator of type I interferon (IFN) -dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Synonyms

Interferon regulatory factor 3, IRF-3, IRF3

Gene Name

IRF3

UniProt

Q14653

Host

Rabbit

Reactivity

Human

Immunogen

This IRF3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from human IRF3.

Clonality

Polyclonal

Tissue Specificity

Expressed constitutively in a variety of tissues.

Applications

WB

Field of Research

Antiviral Signaling, Host-Virus Interaction, Immunology, Innate Immunity, TLR Signaling

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

47219 Da

Applications Notes

WB: 1:2000

Subcellular Location

Cytoplasm. Nucleus Mitochondrion. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757, PubMed:35922005) . When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757) . Recruited to mitochondria via TOMM70:HSP90AA1 upon Sendai virus infection (PubMed:25609812) .

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.