Anti- (Mouse) Dnmt1 Antibody (Center)

About this product

Boster Bio Anti- (Mouse) Dnmt1 Antibody (Center) (Catalog # M00172-2) . Tested in WB, IHC-P, IF application (s) . This antibody reacts with Human, Mouse.

Specifications

Catalog number

M00172-2-80ul

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Synonyms

DNA (cytosine-5) -methyltransferase 1, Dnmt1, Met-1, DNA methyltransferase MmuI, DNA MTase MmuI, MMmuI, MCMT, Dnmt1, Dnmt, Met1, Uim

Gene Name

Dnmt1

UniProt

P13864

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This MouseDnmt1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 881-914 amino acids from the Central region of mouse Dnmt1.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is expressed in embryonic stem cells and in somatic tissues. Isoform 2 is expressed in oocytes, preimplantation embryos, testis and in skeletal muscle during myogenesis

Applications

WB, IHC-P, IF

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS- mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (By similarity) . Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (By similarity) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (By similarity) . Promotes tumor growth (By similarity) .

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

183189 Da

Subcellular Location

Nucleus. Cytoplasm. Note=It is nucleoplasmic through most of the cell cycle and associates with replication foci during S-phase. In germ cells, spermatogonia, preleptotene and leptotene spermatocytes all express high levels of nuclear protein, while the protein is not detected in pachytene spermatocytes, despite the fact they expressed high levels of mRNA. In females, the protein is not detected in non- growing oocytes, in contrast to the growing oocytes. During the growing, the protein is no longer detectable in nuclei but accumulates to very high levels first throughout the cytoplasm. At the time of ovulation, all the protein is cytoplasmic and is actively associated with the oocyte cortex. After fecondation, in the preimplantation embryo, the protein remains cytoplasmic and after implantation, it is exclusively nuclear in all tissue types. Isoform 2 is sequestered in the cytoplasm of maturing oocytes and of preimplantation embryos, except for the 8-cell stage, while isoform 1 is exclusively nuclear

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.