- Catalog number
- M00226-80ul
- Size / count
- 80 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs) -mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
- Synonyms
- Tyrosine-protein kinase receptor UFO, AXL oncogene, AXL, UFO
- Gene Name
- AXL
- UniProt
- P30530
- Host
- Rabbit
- Reactivity
- Human, Rat
- Immunogen
- This AXL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 838-872 amino acids from the C-terminal region of human AXL.
- Clonality
- Polyclonal
- Tissue Specificity
- Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
- Applications
- WB
- Field of Research
- Viral process, Host-Virus Interaction, Innate Immunity, Immunology
- Purification
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- Function
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs) -mediated innate immune response.
- Storage Conditions
- Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
- Observed Molecular Weight
- 98337 Da
- Applications Notes
- WB: 1:2000
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein
- Isotype
- Rabbit IgG
- Contents
- Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
