Anti-MITF Antibody (Monoclonal, ABM1H91)

About this product

Boster Bio Anti-MITF Antibody (Monoclonal, ABM1H91) (Catalog# M00269) . Tested in IHC, WB application (s) . This antibody reacts with Mouse, Human.

Specifications

Catalog number

M00269

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32

Gene Name

MITF

UniProt

O75030

Host

Mouse

Reactivity

Mouse, Human

Immunogen

A partial length recombinant MITF protein (amino acids 150-427)

Clonality

Monoclonal

Clone

Clone: ABM1H91

Tissue Specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE) . Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

Applications

IHC, WB

Field of Research

2339, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Immunology, Innate Immunity, Transcription

Purification

Protein G Chromatography

Concentration

0.5-1mg/mL, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. .

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Observed Molecular Weight

58795 MW

Applications Notes

Western blot: 2-4 μg/ml, IHC: 10 μg/ml

Gene Name Synonym

Microphthalmia-associated transcription factor

Subcellular Location

Nucleus.

Protein Name

Microphthalmia-associated transcription factor

Isotype

IgG1 Kappa

Contents

100 μ g in 200 μ l PBS containing 0.05% BSA and 0.05% sodium azide.