Anti-Hu CD24 Purified Monoclonal Antibody

About this product

Boster Bio Anti-Hu CD24 Purified Monoclonal Antibody (Catalog# M00373) . Tested in Flow Cytometry, IHC-F, MC (CyTOF) application (s) . This antibody reacts with Human.

Specifications

Catalog number

M00373

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Synonyms

HSA, nectadorin, SCLC4

Gene Name

CD24

UniProt

P25063

Host

Mouse

Reactivity

Human

Immunogen

Glycoproteins purified from human NALM-1 cell line. The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.

Clonality

Monoclonal

Clone

Clone: SN3

Tissue Specificity

B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

Applications

Flow Cytometry, IHC-F, CyTOF

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/mL

Form

Liquid

Function

May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells (PubMed:11313396) . In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity (By similarity) .

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

8083 MW

Gene Name Synonym

CD24 molecule

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

Signal transducer CD24

Isotype

Mouse IgG1

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide