Anti-Mouse IL-17A Monoclonal Antibody

About this product

Boster Bio Anti-Mouse IL-17A Monoclonal Antibody (Catalog # M00421) . Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.

Specifications

Catalog number

M00421

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F) . Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and mouse IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Synonyms

IL-17A, interleukin17A, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

Gene Name

IL17A

UniProt

Q62386

Host

Rat

Reactivity

Mouse

Immunogen

This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.

Clonality

Monoclonal

Clone

Clone: Clone: 20B4.G10.F5 IgG2a kappa

Tissue Specificity

Restricted to activated memory T-cells.

Applications

ELISA, Flow Cytometry, WB

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Purification

This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.

Concentration

2.94 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store IL-17A antibody at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

17504 MW

Gene Name Synonym

Interleukin-17A

Subcellular Location

Secreted.

Isotype

IgG2a kappa

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide