Anti-LMNA Antibody

About this product

Boster Bio Anti-LMNA Antibody (Catalog # M00438-4) . Tested in WB application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00438-4-200ul

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.

Synonyms

Prelamin-A/C, Lamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1

Gene Name

LMNA

UniProt

P02545

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This antibody is generated from a mouse immunized with a recombinant protein.

Clonality

Monoclonal

Clone

Clone: 1073CT3.1.3

Tissue Specificity

In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.

Applications

WB

Field of Research

Cell Biology, Developmental Biology, Signal Transduction

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

74139 Da

Applications Notes

WB: 1:4000

Subcellular Location

Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Nucleus matrix. Note=Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleavage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina (PubMed:15317753) EMD is required for proper localization of non-farnesylated prelamin- A/C (PubMed:19323649) .

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.