Anti-FHIT Antibody

About this product

Boster Bio Anti-FHIT Antibody (Catalog # M01200-1) . Tested in WB, Flow Cytometry application (s) . This antibody reacts with Human.

Specifications

Catalog number

M01200-1-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cleaves P (1) -P (3) -bis (5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P (1) -P (4) -bis (5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P (1) -P (3) -bis (5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca (2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

Synonyms

Bis (5'-adenosyl) -triphosphatase, AP3A hydrolase, AP3Aase, Diadenosine 5',5'''-P1, P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FHIT

Gene Name

FHIT

UniProt

P49789

Host

Mouse

Reactivity

Human

Immunogen

This FHIT antibody is generated from a mouse immunized with a recombinant protein.

Clonality

Monoclonal

Clone

Clone: 1093CT23.1.2

Tissue Specificity

Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung Phospho-FHIT undetected in all tested human tumor cell lines

Applications

WB, Flow Cytometry

Field of Research

Mitochondria

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

16858 Da

Subcellular Location

Cytoplasm. Mitochondrion. Nucleus

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.