Anti-Vinculin Antibody

About this product

Boster Bio Anti-Vinculin Antibody (Catalog # M01207-2) . Tested in WB, Flow Cytometry, IHC-P application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01207-2-50ul

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity) .

Synonyms

Vinculin, Metavinculin, Vcl

Gene Name

Vcl

UniProt

Q64727

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 903-937 amino acids from mouse.

Clonality

Polyclonal

Applications

WB, Flow Cytometry, IHC-P

Field of Research

Cardiovascular, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Actin filament (F-actin) -binding protein involved in cell- matrix adhesion and cell-cell adhesion. Regulates cell-surface E- cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity) .

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

116717 Da

Subcellular Location

Cell membrane {ECO:0000250|UniProtKB:P12003}; Peripheral membrane protein {ECO:0000250|UniProtKB:P12003}; Cytoplasmic side {ECO:0000250|UniProtKB:P12003}. Cell junction, adherens junction {ECO:0000250|UniProtKB:P12003}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:P12003}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P85972}. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cell projection, podosome. Note=Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions {ECO:0000250|UniProtKB:P12003}

Prediction Reactivity

Rat

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.