Anti-CARD9 Antibody (N-term)

About this product

Boster Bio Anti-CARD9 Antibody (N-term) (Catalog # M01410) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

M01410-50ul

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM- tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity) . Controls CLEC7A (dectin-1) -mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2) -induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells.

Synonyms

Caspase recruitment domain-containing protein 9, hCARD9, CARD9

Gene Name

CARD9

UniProt

Q9H257

Host

Mouse

Reactivity

Human

Immunogen

This CARD9 antibody is generated from a mouse immunized with a recombinant protein from human CARD9.

Clonality

Monoclonal

Clone

Clone: 2156CT20.4.85

Tissue Specificity

Expression is restricted to several populations of phagocytes, such as macrophages, monocytes, and dendritic cells (PubMed:33548172) . Highly expressed in spleen (PubMed:11053425) . Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain (PubMed:11053425) .

Applications

WB

Field of Research

Cell Biology

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

62241 Da

Applications Notes

WB: 1:500

Subcellular Location

Cytoplasm

Prediction Reactivity

Human

Isotype

IgG1, κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.