Anti-RAB11A Antibody

About this product

Boster Bio Anti-RAB11A Antibody (Catalog # M01436-1) . Tested in WB, IHC-P application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01436-1-200ul

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.

Synonyms

Ras-related protein Rab-11A, Rab-11, YL8, RAB11A, RAB11

Gene Name

RAB11A

UniProt

P62491

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This RAB11A antibody is generated from a mouse immunized with a purified recombinant protein of human RAB11A.

Clonality

Monoclonal

Clone

Clone: 1565CT164.36.80

Applications

WB, IHC-P

Field of Research

Cancer, Signal Transduction

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

24394 Da

Subcellular Location

Cell membrane; Lipid-anchor. Endosome membrane. Recycling endosome membrane; Lipid-anchor. Cleavage furrow. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle membrane. Golgi apparatus. Golgi apparatus, trans-Golgi network. Note=Localized to WDR44-positive endosomes and tubules (PubMed:32344433) . Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane (PubMed:11994279) . During interphase, localized in vesicles continuously moving from peripheral sorting endosomes towards the pericentrosomal ERC (PubMed:20026645) . Localizes to the cleavage furrow (PubMed:15601896) . Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297) Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211) . Localized to rhodopsin transport carriers when interacting with RAB11AFIP3 and ASAP1 in photoreceptors (PubMed:25673879) .

Isotype

IgG1, κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.