Anti-RPTOR Antibody

About this product

Boster Bio Anti-RPTOR Antibody (Catalog # M01463-1) . Tested in IHC-P, WB application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01463-1-80ul

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E) . mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Synonyms

Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR) -scaffold protein, RPTOR, KIAA1303, RAPTOR

Gene Name

RPTOR

UniProt

Q8N122

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This RPTOR antibody is generated from a mouse immunized with recombinant protein.

Clonality

Monoclonal

Clone

Clone: 1411CT316.2.151.34

Tissue Specificity

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta

Applications

IHC-P, WB

Field of Research

Cell Biology, Signal Transduction, Lysosome

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

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Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

149038 Da

Subcellular Location

Lysosome membrane. Cytoplasm Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability: recruited to lysosome membranes via interaction with GTP-bound form of RagA/RRAGA (or RagB/RRAGB) in complex with the GDP-bound form of RagC/RRAGC (or RagD/RRAGD), promoting recruitment of mTORC1 to the lysosomes (PubMed:31601764, PubMed:31601708) . In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased (PubMed:23953116) .

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.