Anti-CARM1 (C-terminus) monoclonal Antibody

About this product

Boster Bio Anti-CARM1 (C-terminus) monoclonal Antibody catalog # M01486. Tested in WB, IP applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

M01486

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Histone-arginine methyltransferase CARM1;2.1.1.319

Gene Name

CARM1

UniProt

Q86X55

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to the C-terminus of Human CARM1.

Clonality

Monoclonal

Tissue Specificity

Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Applications

WB, IP

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

65854 MW

Gene Name Synonym

Histone-arginine methyltransferase CARM1

Subcellular Location

Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.

Protein Name

Histone-arginine methyltransferase CARM1

Contents

1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2