Anti-EPHA5 Antibody

About this product

Boster Bio Anti-EPHA5 Antibody (Catalog # M01494-1) . Tested in WB, IHC-P application (s) . This antibody reacts with Human.

Specifications

Catalog number

M01494-1-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Ephrin type-A receptor 5, Brain-specific kinase, EPH homology kinase 1, EHK-1, EPH-like kinase 7, EK7, hEK7, EPHA5, BSK, EHK1, HEK7, TYRO4

Gene Name

EPHA5

UniProt

P54756

Host

Mouse

Reactivity

Human

Immunogen

Purified His-tagged EPHA5 protein (Fragment) was used to produced this monoclonal antibody.

Clonality

Monoclonal

Clone

Clone: 46CT61.6.4

Tissue Specificity

Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord

Applications

WB, IHC-P

Field of Research

Cardiovascular, Neuroscience, Signal Transduction

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity) .

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

114803 Da

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, axon {ECO:0000250|UniProtKB:P54757}. Cell projection, dendrite

Isotype

IgG1κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.