Anti-LAT Purified Monoclonal Antibody

About this product

Boster Bio Anti-LAT Purified Monoclonal Antibody (Catalog# M01654) . Tested in WB, IP, Flow Cytometry application (s) . This antibody reacts with Human.

Specifications

Catalog number

M01654

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.

Synonyms

IMD52

Gene Name

LAT

UniProt

O43561

Host

Mouse

Reactivity

Human

Immunogen

Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT. The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

Clonality

Monoclonal

Clone

Clone: LAT-01

Tissue Specificity

Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level) .

Applications

WB, IP, Flow Cytometry

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/mL

Form

Liquid

Function

Required for TCR (T-cell antigen receptor) - and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III) -mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

27930 MW

Gene Name Synonym

Linker for activation of T cells

Subcellular Location

Cell membrane

Protein Name

Linker for activation of T-cells family member 1

Isotype

Mouse IgG1

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide