Anti-PDK4 Antibody

About this product

Boster Bio Anti-PDK4 Antibody (Catalog # M02132) . Tested in WB application (s) . This antibody reacts with Human, Mouse.

Specifications

Catalog number

M02132-80ul

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

Synonyms

[Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4

Gene Name

PDK4

UniProt

Q16654

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Purified His-tagged PDK4 protein (Fragment) was used to produced this monoclonal antibody.

Clonality

Monoclonal

Clone

Clone: 345CT19.6.2

Tissue Specificity

Ubiquitous; highest levels of expression in heart and skeletal muscle.

Applications

WB

Field of Research

Metabolism, Signal Transduction, Mitochondria

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

46469 Da

Applications Notes

WB: 1:250

Subcellular Location

Mitochondrion matrix.

Isotype

IgG1

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.