Anti-Ms CD8a Purified Monoclonal Antibody

About this product

Boster Bio Anti-Ms CD8a Purified Monoclonal Antibody (Catalog# M02236) . Tested in Flow Cytometry, IP, IHC-P, IHC-F, ICC application (s) . This antibody reacts with Mouse.

Specifications

Catalog number

M02236

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Synonyms

Ly-2, Ly-B, Ly-35, Lyt-2, BB154331

Gene Name

CD8A

UniProt

P01731

Host

Rat

Reactivity

Mouse

Immunogen

Murine spleen cells. The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen) .

Clonality

Monoclonal

Clone

Clone: 53-6.7

Applications

Flow Cytometry, IP, IHC-P, IHC-F, ICC

Purification

Purified by protein-G affinity chromatography.

Concentration

1 mg/mL

Form

Liquid

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs) . In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs) . This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK- cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

27456 MW

Gene Name Synonym

CD8a molecule

Subcellular Location

Cell membrane

Protein Name

T-cell surface glycoprotein CD8 alpha chain

Isotype

Rat IgG2a kappa

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide