Anti-TFIIH p89 ERCC3 Monoclonal Antibody

About this product

Boster Bio Anti-TFIIH p89 ERCC3 Monoclonal Antibody catalog # M03103. Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Bovine, Dog.

Specifications

Catalog number

M03103

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

General transcription and DNA repair factor IIH helicase subunit XPB; TFIIH subunit XPB;3.6.4.12; Basic transcription factor 2 89 kDa subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH 89 kDa subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein; ERCC3; XPB, XPBC

Gene Name

ERCC3

UniProt

P19447

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine, Dog

Immunogen

Purified recombinant human TFIIH p89 (C-terminus) protein fragments expressed in E.coli.

Clonality

Monoclonal

Applications

WB

Purification

Affinity purification

Concentration

1 mg/mL

Form

Liquid

Function

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP- dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

89278 MW

Gene Name Synonym

General transcription and DNA repair factor IIH helicase subunit XPB

Subcellular Location

Nucleus.

Protein Name

General transcription and DNA repair factor IIH helicase subunit XPB

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.