Anti-TRIM33 (C-terminus) monoclonal Antibody

About this product

Boster Bio Anti-TRIM33 (C-terminus) monoclonal Antibody catalog # M03133. Tested in WB applications. This antibody reacts with Human.

Specifications

Catalog number

M03133

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase TRIM33;2.3.2.27; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; RING-type E3 ubiquitin transferase TRIM33 ; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; TRIM33; KIAA1113, RFG7, TIF1G

Gene Name

TRIM33

UniProt

Q9UPN9

Host

Mouse

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to the C-terminus of Human TRIM33.

Clonality

Monoclonal

Tissue Specificity

Expressed in stem cells at the bottom of the crypts of the colon (at protein level) . Expressed in colon adenomas and adenocarcinomas (at protein level) . Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

Applications

WB

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity) . Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

122533 MW

Applications Notes

WB: 1:1000

Gene Name Synonym

E3 ubiquitin-protein ligase TRIM33

Subcellular Location

Nucleus

Protein Name

E3 ubiquitin-protein ligase TRIM33

Contents

1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2