Anti-Mesothelin MSLN Monoclonal Antibody

About this product

Boster Bio Anti-Mesothelin MSLN Monoclonal Antibody (Catalog # M03266) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M03266

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Synonyms

Mesothelin, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen

Gene Name

MSLN

UniProt

Q13421

Host

Mouse

Reactivity

Human

Immunogen

This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.

Clonality

Monoclonal

Clone

Clone: Clone: MN-1 IgG2a

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) .

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Cancer, Cell Type Markers, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Purification

This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross-reactivity with homologues from other sources has not been tested.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Membrane-anchored forms may play a role in cellular adhesion.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

68986 MW

Gene Name Synonym

Mesothelin

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.

Isotype

IgG2a

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide