Anti-PAG1 Purified Monoclonal Antibody

About this product

Boster Bio Anti-PAG1 Purified Monoclonal Antibody (Catalog# M03711) . Tested in IP, WB, IHC-P application (s) . This antibody reacts with Rat, Cow, Human, Mouse.

Specifications

Catalog number

M03711

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Synonyms

CBP, PAG

Gene Name

PAG1

UniProt

Q3U1F9

Host

Mouse

Reactivity

Rat, Cow, Human, Mouse

Immunogen

C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Clonality

Monoclonal

Clone

Clone: PAG-C1

Tissue Specificity

Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level) .

Applications

IP, WB, IHC-P

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/mL

Form

Liquid

Function

Negatively regulates TCR (T-cell antigen receptor) - mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

46981 MW

Gene Name Synonym

Phosphoprotein membrane anchor with glycosphingoli

Subcellular Location

Cell membrane

Protein Name

Phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Isotype

Mouse IgG2b

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide