Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody

About this product

Boster Bio Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (Catalog# M03989) . Tested in WB, IHC-P, ICC, IP, Flow Cytometry application (s) . This antibody reacts with Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast, Arabidopsis.

Specifications

Catalog number

M03989

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs) . The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs) . The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –; this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes) . Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.

Synonyms

TUBA

Gene Name

TUBA1

UniProt

Q71U36

Host

Mouse

Reactivity

Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast, Arabidopsis

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.

Clonality

Monoclonal

Clone

Clone: TU-01

Tissue Specificity

Expressed at a high level in fetal brain.

Applications

WB, IHC-P, ICC, IP, Flow Cytometry

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/mL

Form

Liquid

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

50136 MW

Gene Name Synonym

Tubulin alpha 1

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Name

Tubulin alpha-1A chain

Isotype

Mouse IgG1

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide