Anti-RAD9A monoclonal Antibody

About this product

Boster Bio Anti-RAD9A monoclonal Antibody catalog # M04161. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Specifications

Catalog number

M04161

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cell cycle checkpoint control protein RAD9A; hRAD9;3.1.11.2; DNA repair exonuclease rad9 homolog A; RAD9A

Gene Name

RAD9A

UniProt

Q99638

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant full length Human RAD9A.

Clonality

Monoclonal

Applications

WB, IP

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) . The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

42547 MW

Gene Name Synonym

Cell cycle checkpoint control protein RAD9A

Subcellular Location

Nucleus

Protein Name

Cell cycle checkpoint control protein RAD9A

Contents

1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2