Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298

About this product

Boster Bio Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (Catalog # M04387) . Tested in IHC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M04387

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2S1; EIF2A

Gene Name

EIF2S1

UniProt

P05198

Host

Rabbit

Reactivity

Human

Cross Reactivity

Reacts to human eIF-2a (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. No cross-reactivity to eIF-2a without phosphorylation at Ser51. May also react to mouse or rat Phospho-eIF-2a (Ser51) .

Immunogen

A phospho-peptide corresponding to human Phospho- eIF-2a (Ser51)

Clonality

Monoclonal

Clone

Clone: RM298

Applications

IHC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing, Translation

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/mL, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2- GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

36112 MW

Gene Name Synonym

Eukaryotic translation initiation factor 2 subunit 1

Subcellular Location

Cytoplasm, Stress granule. Colocalizes with NANOS3 in the stress granules.

Prediction Reactivity

Mouse, Rat

Isotype

Rabbit IgG

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide