Anti-CENP-E Monoclonal Antibody

About this product

Boster Bio Anti-CENP-E Monoclonal Antibody (Catalog # M04553) . Tested in IF, IP, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M04553

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

2

Synonyms

CENP-E, Centromere-associated protein E, Centromeric protein E, KIF10, Kinesin-7, Kinesin family member 10, Kinesin-related protein CENPE

Gene Name

CENPE

UniProt

Q02224

Host

Mouse

Reactivity

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.

Clonality

Monoclonal

Clone

Clone: Clone: 1H12 IgG1

Applications

ELISA, IF, IP, WB

Field of Research

Cell Cycle, Chromosome Structure, Epigenetics and Nuclear Signaling

Purification

This Protein A purified antibody is directed against human CENP-E protein. The product was purified from tissue culture supernatant by chromatography. This antibody reacts with CENP-E from human cells. Reactivity against homologues from other sources is not known.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

316415 MW

Gene Name Synonym

Centromere-associated protein E

Subcellular Location

Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

Isotype

IgG1

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide