Anti-BHLH3 Antibody (N-term)

About this product

Boster Bio Anti-BHLH3 Antibody (N-term) (Catalog # M04668) . Tested in WB application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M04668-200ul

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) . Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.

Synonyms

Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, bHLHb3, Differentially expressed in chondrocytes protein 2, hDEC2, Enhancer-of-split and hairy-related protein 1, SHARP-1, BHLHE41, BHLHB3, DEC2, SHARP1

Gene Name

BHLHE41

UniProt

Q9C0J9

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This BHLH3 antibody is generated from a mouse immunized with a recombinant protein of human BHLH3.

Clonality

Monoclonal

Clone

Clone: 2135CT82.1.84

Tissue Specificity

Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney

Applications

WB

Field of Research

Cancer, Neuroscience

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

1127894814672706151931441556078218411297197865582508301325083013

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

50498 Da

Applications Notes

WB: 1:4000

Subcellular Location

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}

Prediction Reactivity

Human, Mouse, Rat

Isotype

IgG2b, κ

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.