Anti-GAPDHS Purified Monoclonal Antibody

About this product

Boster Bio Anti-GAPDHS Purified Monoclonal Antibody (Catalog# M06121) . Tested in WB, ICC, Flow Cytometry application (s) . This antibody reacts with Pig, Human.

Specifications

Catalog number

M06121

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Synonyms

SGAPDH, HSD-35

Gene Name

GAPDHS

UniProt

O14556

Host

Mouse

Reactivity

Pig, Human

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Clonality

Monoclonal

Clone

Clone: Hs-8

Tissue Specificity

Testis specific.

Applications

WB, ICC, Flow Cytometry

Purification

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .

Concentration

1 mg/mL

Form

Liquid

Function

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity) . .

Storage Conditions

Store at 2-8°C. Do not freeze.

Observed Molecular Weight

44501 MW

Gene Name Synonym

Glyceraldehyde-3-phosphate dehydrogenase, spermato

Subcellular Location

Cytoplasm.

Protein Name

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

Isotype

Mouse IgM

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide