Anti-Monomethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM140

About this product

Boster Bio Anti-Monomethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM140 (Catalog # M06819-3) . Tested in WB, ChIP, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.

Specifications

Catalog number

M06819-3

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Histone H3.3; H3F3A; H3.3A, H3F3; PP781; H3F3B; H3.3B

Gene Name

H3F3A

UniProt

P84243

Host

Rabbit

Reactivity

Human, Vertebrates

Cross Reactivity

This antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1) . No cross-reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3

Immunogen

A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)

Clonality

Monoclonal

Clone

Clone: RM140

Applications

WB, ChIP, ICC, ELISA, Luminex

Field of Research

2339, Epigenetics and Nuclear Signaling, Histones

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

1 mg/mL

Form

Liquid

Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

15328 MW

Gene Name Synonym

Histone H3.3

Subcellular Location

Nucleus. Chromosome.

Isotype

Rabbit IgG

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide