Anti-H3f3b Antibody (C-Term)

Catalog number M06819-30-200ul

Price for M06819-30-200ul

Size / count 200 µL

About this product

Boster Bio Anti-H3f3b Antibody (C-Term) (Catalog # M06819-30) . Tested in WB application (s) . This antibody reacts with Mouse.

Specifications

Catalog number: M06819-30-200ul
Size / count: 200 µL
Dry ice: No
Hazardous: No
Discontinued: No
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms: Histone H33, H3f3a, H33a
Gene Name: H3-3a
UniProt: P84244
Host: Rabbit
Reactivity: Mouse
Immunogen: This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-136 amino acids from mouse H3f3b.
Clonality: Polyclonal
Applications: WB
Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Storage Conditions: Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight: 15328 Da
Applications Notes: WB: 1:2000
Subcellular Location: Nucleus. Chromosome.
Isotype: Rabbit IgG
Contents: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Frequently Asked Questions

What is the catalog number for Anti-H3f3b Antibody (C-Term)?

The catalog number for Anti-H3f3b Antibody (C-Term) is M06819-30-200ul.

What sizes are available for Anti-H3f3b Antibody (C-Term)?

Anti-H3f3b Antibody (C-Term) is available in the following size(s): 200 µL.

Is Anti-H3f3b Antibody (C-Term) in stock?

Anti-H3f3b Antibody (C-Term) is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-H3f3b Antibody (C-Term)?

The safety data sheet for Anti-H3f3b Antibody (C-Term) is available here: https://www.bosterbio.com/msds?sku=M06819-30.

What is Anti-H3f3b Antibody (C-Term) used for?

Boster Bio Anti-H3f3b Antibody (C-Term) (Catalog # M06819-30) . Tested in WB application (s) . This antibody reacts with Mouse.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.