Anti-TFIIB monoclonal GTF2B Antibody

About this product

Boster Bio Anti-TFIIB monoclonal GTF2B Antibody catalog # M06893. Tested in WB, IHC, ICC applications. This antibody reacts with Human.

Specifications

Catalog number

M06893

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Transcription initiation factor IIB

Gene Name

GTF2B

UniProt

Q00403

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full length Human TFIIB.

Clonality

Monoclonal

Tissue Specificity

Expressed in the inner cell mass forming the embryoblast (PubMed:24441171) . Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171) .

Applications

WB, IHC, ICC

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II) . Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682) . Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312) . Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352) . Associates with chromatin to core promoter- specific regions (PubMed:12931194, PubMed:24441171) . Binds to two distinct DNA core promoter consensus sequence elements in a TBP- independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841) . Modulates transcription start site selection (PubMed:10318856) . Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34833 MW

Gene Name Synonym

Transcription initiation factor IIB

Subcellular Location

Nucleus

Protein Name

Transcription initiation factor IIB

Contents

Mouse IgG2b, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2