Anti-ULK3 Antibody (C-term)

About this product

Boster Bio Anti-ULK3 Antibody (C-term) (Catalog # M07706-1) . Tested in WB application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M07706-1-80ul

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) . Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Synonyms

Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3

Gene Name

ULK3

UniProt

Q6PHR2

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This ULK3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 435-468 amino acids from the C-terminal region of human ULK3.

Clonality

Monoclonal

Clone

Clone: 1404CT148.89.25

Tissue Specificity

Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord

Applications

WB

Field of Research

Signal Transduction, Autophagy

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) . Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

53444 Da

Subcellular Location

Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence

Isotype

IgG1, k

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.