Human Cystatin B Recombinant Protein

About this product

Human Cystatin B Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-98aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Specifications

Catalog number

PROTP04080-250ug

Size / count

250 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cystatin-B, Stefin B, PME, CST6

Gene Name

CSTB

UniProt

P04080

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Neuroscience

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

13 kDa (118aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl