Human Enolase 1/Non-neural enolase Recombinant Protein

About this product

Human Enolase 1/Non-neural enolase Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-434aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Specifications

Catalog number

PROTP06733-500ug

Size / count

500 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1, ENO1L1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, MBP1, MBPB1, MPB1, MYC promoter-binding protein 1, NNE, Non-Neuronal Enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH, Tau-crystallin

Gene Name

ENO1

UniProt

P06733

Tag

Non-Tagged

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Metabolism

Purification

> 90% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 20,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

47.1 kDa (434aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol