Mouse IL-1ra/IL-1F3 Recombinant Protein

About this product

Mouse IL-1ra/IL-1F3 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 27-178aa. Application (s) : SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Specifications

Catalog number

PROTP25085-250ug

Size / count

250 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RA

Gene Name

Il1rn

UniProt

P25085

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Bioactivity

Field of Research

Hormones and Cytokines

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/mL with Mouse IL-1 alpha.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

20 kDa (177aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol