Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein

About this product

Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 30-207aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Specifications

Catalog number

PROTP30405-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cyclophilin D, Cyclophilin F, CYP3, CYPD, hCyP3, Mitochondral, Peptidyl prolyl cis trans isomerase, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F Cyclophilin 3, PPIase, PPIF, Rotamase

Gene Name

PPIF

UniProt

P30405

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Enzymes and Proteases

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

21.1 kDa (199aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol