Human recombinant BMP-4 (Bone morphogenetic protein-4) protein, AF

About this product

Bone Morphogenetic Protein-4 (BMP-4) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-4 can bind with the TGF-β receptor and trigger SMAD protein signal transduction. BMP-4 has a significant expression in the prostate, it plays a vital role in the development of mesoderm into bones and the development of the epidermis and Dorsal-Ventral axis.

Specifications

Catalog number

PROTP12644-5-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

BMP-2B, DVR4

Gene Name

BMP4

UniProt

P12644

Reactivity

Human

Tag

His Tag (C-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>95% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <0.58 ng/mL.

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium carbonate, pH 9.0. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 12.88 kDa. The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

AA Sequence

MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR with polyhistidine tag at the C-terminus.